S15-DKO (NSG&reg;-SGM3-IL15-MHC I/II DKO) mice combine the features of the highly immunodeficient NSG mouse (deficient in B, T, and NK cells as well as C5 complement) with an MHC class I/II double knock-out and the expression of human cytokines IL3 (interleukin 3), CSF2 (colony stimulating factor 2; GM-CSF), KITLG (KIT ligand; SCF), and IL15 (interleukin 15) that support the stable engraftment of myeloid lineages, regulatory T cell populations and NK cells. S15-DKO is the next iteration of the NSG-MHC I/II DKO mouse (Stock No. <a href="https://www.jax.org/strain/025216">025216</a>) with genetic modifications that promote a more comprehensive immune cell repertoire following peripheral blood mononuclear cell (PBMC) engraftment, including myeloid, natural killer, gamma delta T, and mature B cells. This model enables researchers to evaluate therapies such as T cell engagers or CAR T in a more complex human immune model and allows for cell engagement of immune cell subtypes such as myeloid or memory B cells that were not possible in previous cell humanized mouse models. This addresses a major unmet need within the immuno-oncology space. Animals homozygous for all seven of the mutations (Il2rg is X-linked) are viable and fertile, but severely immunocompromised. Maintenance under specific pathogen-free conditions promotes overall colony health. If maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is recommended. Also see additional <a href="https://www.jax.org/jax-mice-and-services/find-mice/nsg-portfolio/housing-and-breeding-considerations-for-nsg-mice">NSG housing information</a>. Enrichment items (e.g., Shepherd Shacks, nesting materials) and reduced housing density may reduce any aggressive behaviors.

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S15-DKO&nbsp;(NSG&reg;-SGM3-IL15-MHC I/II DKO) mice combine the features of the highly immunodeficient NSG mouse (deficient in B, T, and NK cells as well as C5 complement) with an MHC class I/II double knock-out and the expression of human cytokines (IL3, CSF2, KITLG, and IL15) that support the stable engraftment of myeloid lineages, regulatory T cell populations and NK cells. Peripheral blood mononuclear cell (PBMC)-humanized NSG S15-DKO mice (Stock No. <a href="https://www.jax.org/jax-mice-and-services/preclinical-research-services/humanized-mice/pbmc/s15-dko" target="_blank">743720</a>) can be ordered on our <a href="https://www.jax.org/jax-mice-and-services/preclinical-research-services/humanized-mice/humanized-mice-form">humanized mouse order form</a>.

Homozygous for Prkdc<scid>, Homozygous for H2-K1<b-tm1Bpe>, Homozygous for H2-Ab1<g7-em1Mvw>, Homozygous for H2-D1<b-tm1Bpe>, Homozygous for Il2rg<tm1Wjl>, Homozygous for Tg(CMV-IL3,CSF2,KITLG)1Eav, Homozygous for Tg(IL15)1Sz

Homozygous for Prkdc<scid>, Homozygous for H2-K1<b-tm1Bpe>, Homozygous for H2-Ab1<g7-em1Mvw>, Homozygous for H2-D1<b-tm1Bpe>, Hemizygous for Il2rg<tm1Wjl>, Homozygous for Tg(CMV-IL3,CSF2,KITLG)1Eav, Homozygous for Tg(IL15)1Sz