These mice express a 220 amino acid isoform of the human membrane-bound stem cell factor (hSCF or KITLG) in combination with the severe combined immunodeficiency allele (scid) of the protein kinase, DNA activated, catalytic polypeptide (Prkdc) gene and the Il2rgtm1Wjl mutation of the interleukin 2 receptor, gamma chain gene. Newborn NSG-Tg(hu-mSCF) mice engraft human hematopoietic stem cells without pre-conditioning irradiation treatment and develop mature functional humanized immune systems, capable of rejecting histoincompatible human skin grafts. Differentiated c-Kit+ human mast cells can be detected in bone marrow, spleen, and mucosal lung, gastric, and intestinal tissues of engrafted mutant mice.

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 These NSG-Tg(hu-mSCF) mice provide a supportive microenvironment that is conducive to engraftment of human hematopoietic stem cells and would be useful in studies of human mast cell development and allergic responses.

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NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(PGK1-KITLG*220)441Daw/Sz